Effect of Different Doses of Sea Buckthorn (Hippophae rhamnoides L.) Seed Extract (Oil) on Histology of Islets of Langerhan of Pancreas in Alloxan Induced Diabetic Male Wistar Rats
DOI:
https://doi.org/10.37018/TSYP7935Keywords:
Diabetes, Sea buckthorn seed oil, Alloxan monohydrateAbstract
Background: Sea buckthorn seed oil extract contains saturated and unsaturated fatty acids. Alloxan monohydrate is oxygenated pyrimidine derivative, it produces diabetes in experimental animals. The objectives of this study was to determine the histological changes of sea buckthorn extract (seed oil) on Alloxan induced diabetic β-cells of pancreatic tissue in male Wistar rats.
Method: 32 male Wistar rats were divided into 4 groups: A, B, C and D. Alloxan monohydrate in dose of 130mg/kg body weight was given as a single dose to experimental groups B, C and D. From each group half of the group the rats were sacrificed at day 4 of study and the rest on 25th day after administration of Sea buckthorn seed oil extract.
Results: In comparison with the control group on the 4th day of the sacrifice all the rats in the experimental groups showed a decrease in diameter of islets of Langerhans, number of beta cells and an increase in number of beta cells pyknosis and vacuolization. On the 25th sacrificial day, only high dose Sea buckthorn (4ml) treated group D showed an increased in diameter of islets of Langerhans very close to that of controls while low dose (2ml) treated group C showed late recovery as compared to control group.
Conclusion: The study assesses Sea buckthorn’s (seed oil) effects on serum glucose levels by using glucometer and histological parameters (diameter of islet, beta cell pyknosis, number of inflammatory cells) of islets of Langerhans of pancreas. It focuses on animal models, but human clinical trials are required to validate its efficacy and safety.
Downloads
Published
How to Cite
Issue
Section
License
The Journal of Fatima Jinnah Medical University follows the Attribution Creative Commons-Non commercial (CC BY-NC) license which allows the users to copy and redistribute the material in any medium or format, remix, transform and build upon the material. The users must give credit to the source and indicate, provide a link to the license, and indicate if changes were made. However, the CC By-NC license restricts the use of material for commercial purposes. For further details about the license please check the Creative Commons website. The editorial board of JFJMU strives hard for the authenticity and accuracy of the material published in the journal. However, findings and statements are views of the authors and do not necessarily represent views of the Editorial Board.
