Effectiveness of crystal violet stain for localization of mitotic activity in oral squamous cell carcinoma

  • Aneequa Sajjad Akhtar Saeed Medical and Dental College
  • Muhammad Qasim Raza Al Nafees Medical College, Isra University, Islamabad, Pakitan.
  • Syeda Zaira Sajjad Institute of Public Health, Lahore
  • Ihtesham-ud-Din Qureshi Akhtar Saeed Medical and Dental College
  • Syed Sajjad Sarwar Institute of Public Health, Lahore
  • Sadia Minhas Akhtar Saeed Medical and Dental College
Keywords: Crystal violet stain, oral squamous cell carcinoma, mitotic figures

Abstract

Background: Mitotic figure counting is simplest and oldest method for determining proliferative activity of cell. It is considered as one of the important diagnostic aid in cancer pathology. Though advanced methods to evaluate dysplastic features are more precise and definite but expense and time makes them less practicable for routine use. Therefore an effort was made to use economical as well as simple approach involving crystal violet stain (1%) to study the mitotic figures in oral squamous cell carcinoma.

Materials and Methods: This descriptive research included samples, consisting of thirty three cases of the oral squamous cell carcinoma (OSCC). Representative sections were stained with H&E stain and 1% crystal violet stain respectively. The stained sections were viewed under optical microscope to count mitotic figures for evaluating the effectiveness of 1% crystal violet stain. Data obtained was statistically analyzed by using sample t-test.

Results: There was noteworthy increase in the mean mitotic count among the crystal violet stained sections of OSCC in contrast to the OSCC sections stained with H&E (P = 0.00).

Conclusion:1% Crystal violet stain can be considered as one of the optimum stains to observe the mitotic figure. Practice of staining with 1% crystal violet during routine histopathological procedures will be cost effective and may be used as a selective stain.

Author Biographies

Muhammad Qasim Raza, Al Nafees Medical College, Isra University, Islamabad, Pakitan.

M.B.B.S, Al Nafees Medical College, Isra University, Islamabad, Pakitan, 03336497777

Resident Medical Officer

 

Syeda Zaira Sajjad, Institute of Public Health, Lahore

M.B.B.S, FCPS resident (Community Medicine),Institute of Public Health, Lahore,
Resident Medical Officer

Ihtesham-ud-Din Qureshi, Akhtar Saeed Medical and Dental College

M.B.B.S , DCP, M.Phil. (Morbid Anatomy & Histopathology), Professor of Pathology, Akhtar
Saeed Medical & Dental College, Lahore

Syed Sajjad Sarwar, Institute of Public Health, Lahore

M.B.B.S, M.P.H (Public health), Institute of Public Health, Lahore.

AMS/Additional Registrar Sir Gangaram Hospital,Lahore

Sadia Minhas, Akhtar Saeed Medical and Dental College

B.D.S, Mphil. (Oral Pathology), PhD Scholar, Head of Department , Akhtar Saeed Medical &
Dental College,Lahore,

Published
2020-04-16